Mise-en-scene means everything on the scene eg setting, background, costume etc.
an example of mise-en-scene is from jaws. they use the sharks point of view at the start of the film
and the classic jaws theme song to raise suspense. Another example from jaws is when there around the fire and the boy and girl run away from the fire, this suggest that the are moving from the light and into the darkness. The bars also represent that there isolated and can only go further into the darkness.